Journal article
The first histidine triad motif of PhtD is critical for zinc homeostasis in Streptococcus pneumoniae
BA Eijkelkamp, VG Pederick, CD Plumptre, RM Harvey, CE Hughes, JC Paton, CA McDevitt
Infection and Immunity | AMER SOC MICROBIOLOGY | Published : 2016
DOI: 10.1128/IAI.01082-15
Abstract
Streptococcus pneumoniae is the world's foremost human pathogen. Acquisition of the first row transition metal ion zinc is essential for pneumococcal colonization and disease. Zinc is acquired via the ATP-binding cassette transporter AdcCB and two zinc-binding proteins, AdcA and AdcAII. We have previously shown that AdcAII is reliant upon the polyhistidine triad (Pht) proteins to aid in zinc recruitment. Pht proteins generally contain five histidine (His) triad motifs that are believed to facilitate zinc binding and therefore play a significant role in pneumococcal metal ion homeostasis. However, the importance and potential redundancy of these motifs have not been addressed. We examined the..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
Department of Industry, Innovation, Science, Research and Tertiary Education, Australian Government vertical bar Australian Research Council (ARC) provided funding to James C Paton under grant number DP120101432. Department of Industry, Innovation, Science, Research and Tertiary Education, Australian Government vertical bar Australian Research Council (ARC) provided funding to James C Paton and Christopher McDevitt under grant numbers DP120103957 and DP150101856. Department of Health vertical bar National Health and Medical Research Council (NHMRC) provided funding to James C Paton under grant numbers 565526 and 1071659. Department of Health vertical bar National Health and Medical Research Council (NHMRC) provided funding to Christopher McDevitt under grant numbers 1022240 and 1080784.